A region of the influenza A/NT/60/68 PB2 protein containing an antigenic determinant recognized by murine H-2Dd restricted cytotoxic T lymphocytes
Identifieur interne : 002232 ( Main/Exploration ); précédent : 002231; suivant : 002233A region of the influenza A/NT/60/68 PB2 protein containing an antigenic determinant recognized by murine H-2Dd restricted cytotoxic T lymphocytes
Auteurs : Philip A. Reay [Royaume-Uni] ; Ian M. Jones [Royaume-Uni] ; George G. Brownlee [Royaume-Uni]Source :
- Virology [ 0042-6822 ] ; 1988.
English descriptors
- Teeft :
- Amino, Amino acids, Askonas, Assay, Bennink, Braciale, Brownlee, Chromium release assay, Cytotoxic, Encoding, Epitope, Genes encoding, Gotch, Heterologous, Influenza, Influenza nucleoprotein, Influenza virus, Lymphocyte, Lysis, Major target antigen, Mcmichael, Murine, Nucleoprotein, Peptide, Plasmid, Polyclonal, Polymerase, Polypeptide, Recombinant, Recombinant vaccinia virus, Recombinant vaccinia viruses, Specific lysis, Synthetic peptides, Target antigen, Target cells, Townsend, Transfected, Transfected cell lines, Vaccinia, Vaccinia viruses, Viral, Virus, Western blot analysis, Yewdell.
Abstract
Abstract: We have used a recombinant vaccinia virus to investigate the recognition of the PB2 protein of influenza A/NT/60/ 68(H3N2) by murine polyclonal CTL populations. PB2 is recognized as a major cross-reactive target antigen. Recognition of PB2 is under strict genetic control, since BALB/c(H-2d) but not CBA(H-2k) mice are responders. We also demonstrate, by use of cell lines transfected with individual genes encoding class I molecules of the H-2d haplotype, that recognition of PB2 occurs in conjunction with the H-2Dd but not the H-2Kd or H-2Ld molecules. In contrast, recognition of the nucleoprotein of A/PR/8/34 by BALB/c-derived polyclonal CTL is restricted via the H-2Kd molecule. By using three recombinant vaccinia viruses expressing deleted forms of the PB2 protein we show that at least one epitope of the PB2 protein resides within the amino-terminal 256 amino acids. This approach offers an effective method to map the regions of large proteins containing epitopes recognized by CTL.
Url:
DOI: 10.1016/0042-6822(88)90076-1
Affiliations:
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PB2 is recognized as a major cross-reactive target antigen. Recognition of PB2 is under strict genetic control, since BALB/c(H-2d) but not CBA(H-2k) mice are responders. We also demonstrate, by use of cell lines transfected with individual genes encoding class I molecules of the H-2d haplotype, that recognition of PB2 occurs in conjunction with the H-2Dd but not the H-2Kd or H-2Ld molecules. In contrast, recognition of the nucleoprotein of A/PR/8/34 by BALB/c-derived polyclonal CTL is restricted via the H-2Kd molecule. By using three recombinant vaccinia viruses expressing deleted forms of the PB2 protein we show that at least one epitope of the PB2 protein resides within the amino-terminal 256 amino acids. 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